Ionic α-helical polypeptides toward nonviral gene delivery
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چکیده
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Ionic α-helical polypeptides toward nonviral gene delivery.
The advent of polymeric materials has significantly promoted the development and rapid growth of various technologies in biomedical applications, such as tissue engineering and controlled drug and gene delivery. Water-soluble polypeptides bearing functional side chains and adopting stable secondary structures are a new class of functional polymeric materials of potentially broad applications in...
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Nonviral gene delivery with synthetic cationic polymeric vectors is widely recognized as an attractive alternative to viral gene delivery, which suffers from inherent immunogenicity and various side effects. The transfection efficiency and chemotoxicity of these polymeric vectors are often closely related to the density of their cationic charge. Materials with low charge density usually show lo...
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Polypeptides were the first set of materials considered for use as nonviral gene delivery vectors. With its ability to bind and condense anionic plasmid DNA, cationic poly-l-lysine (PLL) was one of the most well studied of the early gene delivery polypeptides. Unfortunately, as a DNA delivery vector, unmodified PLL suffered from low transfection efficiency. Although there have been tremendous e...
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Water-soluble peptides that adopt stable helical conformations are attractive motifs because of their importance in basic science and their broad utility in medicine and biotechnology. Incorporating charged amino-acid residues to improve peptide solubility, however, usually leads to reduced helical stability because of increased side-chain charge repulsion, reduced side-chain hydrophobicity and...
متن کاملTopic Introduction Nonviral Gene Delivery
Modern cell biology increasingly relies on molecular tools to facilitate the study of cellular processes. With the advent of recombinant DNA technology, reverse transcription–polymerase chain reaction (RT-PCR) to amplify almost any gene, and fluorescent proteins that can be fused to any desired target protein, the number and scope of functional studies designed to determine the roles of protein...
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ژورنال
عنوان ژورنال: Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology
سال: 2014
ISSN: 1939-5116
DOI: 10.1002/wnan.1307